| A Study of Epstein-Barr Virus, and Human Leukocyte Antigen Typing in Children with Acute Infectious Mononucleosis |
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Seung-Hoon Hahn1, Wan-Shik Shin2, Hoon Han3, Jin-Han Kang1 |
1Department of Pediatrics, Medical College, The Catholic University of Korea, Seoul, Korea
2Department of Internal Medicine, Medical College, The Catholic University of Korea, Seoul, Korea
3Department of Microbiology, Medical College, The Catholic University of Korea, Seoul, Korea |
| 급성 전염성 단핵구증 환아에서 Epstein-Barr 바이러스의 감염형과 사람 백혈구 항원형 연구 |
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한승훈1, 신완식2, 한훈3, 강진한1 |
1가톨릭대학교 의과대학 소아과학교실
2가톨릭대학교 의과대학 내과학교실
3가톨릭대학교 의과대학 미생물학교실 |
Correspondence:
Jin-Han Kang, Email: kjhan@olmh.cuk.ac.kr
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| Abstract |
Purpose
: The Epstein-Barr virus(EBV), gamma herpesvirus, is an important pathogen that is widespread around the world. The EBV causes various diseases depending on the geographic location, and on the immunity or the premorbid condition of the person exposed to EBV. To evaluate EBV typing may be the most important step to figure out the pathogenesis of EBV associated diseases, and we need to re-evaluate the pathologic role of human leukocyte antigen(HLA) in developing Epstein-Barr virus associated acute infectious mononucleosis by using newly developed methods.
Methods
: This study included 24 children(age range : 6 to 13 years), serologically confirmed with acute infectious mononucleosis. The control group for the HLA type consisted of 200 age-matched healthy children. To classify HLA I, modified ARMs-PCR was used, while modified PCR-SSOP was utilized in typing of HLA II. Also, we performed EBV typing in study patients by using a one-step PCR.
Results
: The results of HLA types : In HLA class I, HLA-A24 was positive in 69 of 200 healthy children and positive in 14 of 24 patients in the study group(relative risk : 3.5724, chi-square; 5.26, P<0.05). In HLA class II, HLA-DRB1*07 was detected in 18 of 200 healthy children, and eight of 24 patients in the study group(relative risk; 506173, chi-square; 9.73, P<0.01). The results of EBV types : In the research group, 20(83.8%) of 24 patients were shedding type A virus, while 4(16.7%) were type B.
Conclusion
: We conclude that development of infectious mononucleosis may be associated with HLA types, and these results suggest that acute infectious mononucleosis could have hereditary traits. And we confirm that type A EBV is highly prevalent in patients with acute infectious mononucleosis in Korea. Also, our results suggest that further large scale studies, including adult groups, regarding the association between pathogenesis of EBV with HLA-DP or HLA-DQ will be warranted. |
| Key Words:
Epstein-Barr virus, EBV type, HLA, Acute infectious mononucleosis |
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