| DNA Methylation Change of IL-4 Gene from T Cell in Allergic Children |
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Jae Won Oh1, Myung Gul Yum1, Chang Ryul Kim1, In-Joon Seol1, Su A Shin1, Ha Baik Lee1, Se Jin Jang2 |
1Department of Pediatrics, College of Medicine, Hanyang University, Korea
2Department of Pathology, Asan Medical Center, University of Ulsan, College of Medicine, Seoul, Korea |
| 영유아기 아토피 환아에서 말초혈액 T 림프구에서 Interleukin-4 유전자의 DNA 메틸화 변화 |
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오재원1, 염명걸1, 김창렬1, 설인준1, 신수아1, 이하백1, 장세진2 |
1한양대학교 의과대학 소아과학교실
2울산대학교 의과대학 병리학교실 |
Correspondence:
Jae Won Oh, Email: jaewonoh@hanyang.ac.kr
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| Abstract |
Purpose
: An understanding of the immunological process is required if primary prevention of atopic diseases is to be developed in early childhood. But, it is too hard to distinguish atopy from nonatopy under the age of two clinically, because the expression of phenotype and cytokines is vague in early childhood. We evaluated DNA methylation changes at Th2 interleukin-4 gene in peripheral blood from atopic children.
Methods
: We selected 15 allergic children(mild : eight, moderate to severe : seven) and seven normal controls by using family allergy scores and clinical histories. We measured Total IgE and Der f II specific IgE levels and cultured peripheral blood mononuclear cells with Der f II stimulation and extracted DNA from Der f II specific T cells. We examined the change of CpG methylation in DNA from atopic and nonatopic children.
Results
: In T cells from normal children, IL-4 DNA were predominantly methylated; otherwise, CpG demethylation occurred in Der f II specific T cells from allergic children.
Conclusion
: IL-4 DNA methylation changes occurred in T genes from allergic children and DNA methylation assay in early childhood. |
| Key Words:
Methylation , Allergy , Child , T cell |
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