| The relation between angiotensin converting enzyme (ACE) gene
polymorphism and neonatal hyperbilirubinemia in Korea |
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Mi Yeoun Kim1, Jae Myoung Lee1, Ji Sook Kim1, Eun Ryoung Kim1, Hee Jae Lee2, Seo Hyun Yoon3, Joo Ho Chung3 |
1Department of Pediatrics, Sung-Ae General Hospital, Seoul, Korea
2Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon, Korea
3Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon, Korea |
| 한국인 신생아 황달과 안지오텐신 전환효소 유전자의 다형성+B7 |
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김미연1, 이재명1, 김지숙1, 김은령1, 이회제2, 윤서현3, 정주호3 |
1성애병원 소아과
2강원대학 약리학교실
3경희대학교 약리학교실 |
Correspondence:
Eun Ryoung Kim, Email: eunicu@hotmail.com
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| Abstract |
Purpose
: Human angiotensin converting enzyme (ACE) gene shows an insertion/deletion polymorphism in 16 intron, and three genotypes are determined by whether a 287 bp fragment of the DNA is present or not; II, ID and DD genotype. DD genotype has been suggested as a risk factor of chronic nephrotic disease such as IgA nephropathy and diabetic nephropathy, various cardiovascular diseases and several other diseases. ACE activity increases in acute hepatitis, chronic persistent hepatitis, chronic active hepatitis and cirrhosis. On the other hand, patients with fatty livers have normal ACE activity. This study was designed to find out the relation between polymorphsims of the ACE genes and neonatal hyperbilirubinemia in Koreans.
Methods
: The genomic DNA was isolated from 110 full-term Korean neonates who had hyperbilirubinemia with no obvious causes (serum bilirubin 12 mg/dL) and 164 neonates of a control population (serum bilirubin 12 mg/dL). We performed polymerase chain reaction (PCR) to see the allele of the ACE gene. Electrophoresis was done in the PCR products in 1.5 percent agarose gel, and then DNA patterns were directly visualized under ethidium bromide staining.
Results
: ACE genotypes in the hyperbilirubinemia group are as follows; 26.36 percent for II, 53.64 percent for ID, 20.00 percent for DD, 0.532 for I allele and 0.468 for D allele. These distributions were not significantly different from those in the control group; 24.39 percent for II, 51.83 percent for DI, 23.78 percent for DD, 0.503 for I allele and 0.497 for D allele.
Conclusion
: In this study, ACE gene polymorphism was detected in the neonatal hyperbilirubinemia and control group. The most frequent genotype was ID. Our results indicate that the ACE gene polymorphism is not associated with the prevalence of neonatal hyperbilirubinemia in Koreans. |
| Key Words:
ACE (angiotensin converting enzyme) gene , Neonatal hyperbilirubinemia , Polymorphism |
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