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Original Article
Detection of Enterovirus in Patients with Aseptic Meningitis by Reverse Transcription and Polymerase Chain Reaction
Souck Joong Yoon, Sung Jin Hong, Young Hyuk Lee, Min Hee Kim, Kyo Sun Kim, Kyu Hyun Park, Jae Myun Lee, Won Young Lee
Clin Exp Pediatr. 1994;37(9):1226-1234.   Published online September 15, 1994
The aim of this study was to determine the applicability of reverse transcription and polymerase chain reaction(RT/PCR) for routine diagnostic use and for the detection of enteroviral mentigitis, Primers were selected in the highly conserved part of the 5'-non-coding region of the enteroviral genome. Enteroviral RNA was detected by the RT/PCR in routinely collected cerebrospinal fluids(CSF) of patients with aseptic...
Transmission of Hepatitis B Virus from Asymptomatic Carrier Mothers to Their Offspring Using the Polymerase Chain Reaction Technique
Jin Sook Kim, Young Ran Yoon, Mi Kyung Kim, Hye Kyung Han, Mi Ryung Um, Kyung Hoon Choi, Mi Na Lee, In Keol Moon, In Kweon Han
Clin Exp Pediatr. 1994;37(2):231-239.   Published online February 15, 1994
Total 8,341 pregnant women who visited Obstetric clinic Cheil General Hospital from January 1991 to July 1992 were screened for HBsAg. Sixty five women with positive HbsAg who delivered healthy babies during that period and their infants are included in the study. HBsAg, Anti-HBs, Anti-HBc, HBeAg and Anti-HBe were tested by radioimmunoassay and hepatitis B virus DNA were studied by polymerase...
Detection of Cytomegalovirus DNA in Urine Culture Using Polymerase Chain Reaction
Young Mo Sohn, Won Young Lee
Clin Exp Pediatr. 1993;36(5):626-633.   Published online May 15, 1993
Polymerase chain reaction(PCR) amplication was used to detect cytomegalovirus (CMV) in tissue culture from the ruine of newborns and patients who was suspected CMV infection. Synthetic oligonucleotide primer pairs were used to amplify DNA from the magor immediate-early and the phosphoprotein 150 genes of CMV AD 169. Amplified products were detected by gel electrophoresis and by dot-blot hybridization with oligonucleotide...
A Basic Study for Respiratory Syncytial Virus Detection Using Polymerase Chain Reaction
Yong Kweon Kim, Jong Duck Kim
Clin Exp Pediatr. 1992;35(10):1402-1410.   Published online October 15, 1992
Basic experimental approach for rapid and acurate detection of respiratory syncitial virus (RSV) infection at gene level has been performed using polymerase chain reaction (PCR). RSV RNA was extracted by RNAzolB methol from RSV which was cultured in Hep-2 cell line. Of RSV genome having about 8,000 base pairs, base pairs between 3237th and 3522nd were selected to make four...