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Purpose : We intended to observe cell death and apoptotic changes in neurons in organotypic hippocampal slice cultures following oxygen-glucose deprivation (OGD), using propidium iodide (PI) uptake, Fluoro-Jade (FJ) staining, TUNEL staining and immunofluorescent staining for caspase-3.
Methods : The hippocampus of 7-day-old rats was cut into 350 µm slices. The slices were cultured for 10 d (date in vitro, DIV... |
