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Original Article

Injury of neurons by oxygen-glucose deprivation in organotypic hippocampal slice culture

David Chanwook Chung, Kyung Sik Hong, Jihui Kang, Young Pyo Chang

Clin Exp Pediatr. 2008;51(10):1112-1117. Published online October 15, 2008

Purpose : We intended to observe cell death and apoptotic changes in neurons in organotypic hippocampal slice cultures following oxygen-glucose deprivation (OGD), using propidium iodide (PI) uptake, Fluoro-Jade (FJ) staining, TUNEL staining and immunofluorescent staining for caspase-3. Methods : The hippocampus of 7-day-old rats was cut into 350 µm slices. The slices were cultured for 10 d (date in vitro, DIV...

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