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Original Article
Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia
Kyung Nam Koh, Meerim Park, Bo Eun Kim, Ho Joon Im, Chan-Jeoung Park, Seongsoo Jang, Hyun Sook Chi, Jong Jin Seo
Clin Exp Pediatr. 2010;53(11):957-964.   Published online November 30, 2010
Purpose

Our study attempted to determine the prognostic significance of minimal residual disease (MRD) detected by a simplified flow cytometric assay during induction chemotherapy in children with B-cell acute lymphoblastic leukemia (B-ALL).

Methods

A total of 98 patients were newly diagnosed with precursor B-ALL from June 2004 to December 2008 at the Asan Medical Center (Seoul, Korea). Of those, 37 were eligible for...

Single Cell Dissociation Methods for Flow Cytometric Cell Death Analysis of Hypoxia-Ischemia Injured Newborn Rat Pup Brain
Jong Hee Hwang, Dong Kyung Sung, Chang Won Choi, Saem Kang, Yun Sil Chang, Won Soon Park, Munhyang Lee
Clin Exp Pediatr. 2005;48(5):545-550.   Published online May 15, 2005
Purpose : Newborn brain tissue has to be dissociated into a single cell suspension for flow cytometric analysis of cell death during hypoxia-ischemia. Thus the development of a method to dissociate cells from the brain tissue with least damage and maintenance of membrane and antigen integrity remains the challenge for the in vivo application of this technique. We evaluated the...
Characterization of Mutations in Bruton's Tyrosine Kinase(Btk) Gene from Unrelated 3 X-linked Agammaglobulinemia(XLA) Families in Korea
Chang-Hwa Song, Eun-Kyeong Jo, Jeong-Kyu Park, Jung-Soo Kim, Soo-Jong Hong, Jae-Ho Lee
Clin Exp Pediatr. 2002;45(3):302-310.   Published online March 15, 2002
Purpose : X-linked agammaglobulinemia(XLA) is an immunodeficiency caused by abnormalities in Bruton's tyrosine kinase(Btk), and is characterized by a deficiency of peripheral blood B cells. We studied cytoplasmic expression of Btk protein and analyzed the Btk gene in peripheral blood mononuclear cells(PBMC) from three XLA families in Korea. Methods : Heparinized venous blood samples were collected from four XLA patients...
Characterization of Bruton's Tyrosine Kinase Genetic Mutations in One Korean X-linked Agammaglobulinemia Family
Eun-Kyeong Jo, Chang-Hwa Song, Jeong-Kyu Park, Young-Jong Baek, Hye-Young Rhu, Jae-Ho Lee, Tai-Ju Hwang, Hoon Kook
Clin Exp Pediatr. 2002;45(2):183-191.   Published online February 15, 2002
Purpose : X-linked agammaglobulinemia(XLA) is an immunodeficiency caused by abnormalities in Bruton's tyrosine kinase(Btk), and is characterized by a deficiency of peripheral blood B cells. We studied the cytoplasmic expression of Btk protein and analyzed the Btk gene in peripheral blood mononuclear cells from two siblings and one cousin with XLA, as well as additional family members. Methods : Btk...
Protein and Genetic Analysis of Bruton's Tyrosine Kinase(Btk) in Three Korean X-linked Agammaglobulinemia(XLA) Families
Eun-Kyeong Jo, Chang-Hwa Song, Jeong-Kyu Park, Oh Kyung Lee, Dong-Soo Kim
Clin Exp Pediatr. 2002;45(1):44-54.   Published online January 15, 2002
Purpose : Mutations in the Bruton' s tyrosine kinase(Btk) gene are responsible for X-linked agammaglobulinemia( XLA), an immunodeficiency caused by a block in B cell differentiation. In this report we characterize the protein expression and genetic mutations of Btk in four Korean patients with three unrelated XLA families. Methods : The resulting Btk proteins were characterized by a flow cytometry and the mutations were analyzed...
Case Report
A Case of Type Ⅰ Glanzmann's Thrombasthenia Diagnosed by Flow Cytometry
Mun Su Lee, Choong Ho Shin, Kyu Young Kim, Dong Woo Son, Hwa Ryung Chung, Do Hyun Kim
Clin Exp Pediatr. 1999;42(1):133-137.   Published online January 15, 1999
Glanzmann's thrombasthenia is a rare autosomal recessive hemorrhagic disorder of platelet function with missing or abnormal platelet plasma membrane glycoprotein Ⅱb-Ⅲa, which functions as a receptor for fibrinogen. We have experienced a case of thrombasthenia in a 6-year-old female whose chief complaints were easy bruising, frequent epistaxis, arthralgia and swelling of the right ankle joint. Bleeding time was prolonged in...
Original Article
Analysis of Platelet Membrane Glycoprotein Ⅱb-Ⅲa complex in Whole Blood of Glanzmann's Thrombasthenia by Flow Cytometry
Byoung Geun Lee, Man Choon Kang, Jong Man Park, Pyung Han Hwang, Jung Soo Kim
Clin Exp Pediatr. 1994;37(11):1540-1547.   Published online November 15, 1994
Glanzmann's thrompasthenia is a rare autosomal recessive hemorrhagic disorder characterized by prolonged bleeding time, and deficient or absent clot retraction in the presence of normal platelet count. The major underlying abnormality in this disease is grossly defective first-phase aggregation of platelet, which are unresponsive to ADP or other platelet agonists such as epinephrine, collagen, thrombin in any concentration. This disability...
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