Korean Journal of Pediatrics 2005;48(8):871-876.
Published online August 15, 2005.
Tumor Necrosis Factor and Lymphotoxin-α Gene Polymorphism in Korean Children with Type 1 Diabetes
Jin Soon Suh1, So Young Park1, Min Ho Jung1, Byung Kyu Suh1, Tae Gyu Kim1, Byung Churl Lee2
1Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea
2Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea
한국 소아 1형 당뇨병에서 종양괴사인자 및 림프독소-α 유전자 다형성
서진순1, 박소영1, 정민호1, 서병규1, 김태규1, 이병헉2
1가톨릭대학교 의과대학 소아과학교실
2가톨릭대학교 의과대학 미생물학교실
Correspondence: 
Byung Churl Lee, Email: byungcl@catholic.ac.kr
Abstract
Purpose
: Recently, it was reported that tumor necrosis factor(TNF) and lymphotoxin-α(LT-α) gene regions might be a susceptible loci to type 1 diabetes in Japanese. The purpose of this study was to investigate the association of TNF and LT-α gene polymorphisms with disease susceptibility in Korean children with type 1 diabetes.
Methods
: Forty-nine Korean children with type 1 diabetes(29 girls and 20 boys) and 94 healthy Koreans were investigated in this study. Genotyping for -857T/C polymorphism in the TNF promoter region and LT-α gene polymorphism were performed by PCR-RFLP(restriction fragment length polymorphism). TNF promoter -1031C/T polymorphism was detected by allele-specific PCR.
Results
: The distribution of the -857T/C and -1031C/T genotype in the TNF promoter region was not different between diabetic children and the controls. The frequency of TT genotype in the distribution of TNF -1031C/T polymorphism in diabetic children with diabetic ketoacidosis(DKA) at diagnosis was significantly lower than those without DKA(P<0.05). No significant difference in the distribution of LT-α gene polymorphism was observed between diabetic children and the controls. There was no association between clinical characteristics of type 1 diabetes and LT-α gene polymorphisms.
Conclusion
: These results suggest that TNF promoter -857T/C and LT-α gene polymorphisms are not associated with susceptibility to type 1 diabetes in Korean children. TNF promoter -1031C/T polymorphism might be related to clinical manifestations(DKA) of type 1 diabetes.
Key Words: Type 1 diabetes , Gene , Polymorphism , Tumor necrosis factor , Lymphotoxin-α


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