| Tumor Necrosis Factor and Lymphotoxin-α Gene Polymorphism in Korean Children with Type 1 Diabetes |
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Jin Soon Suh1, So Young Park1, Min Ho Jung1, Byung Kyu Suh1, Tae Gyu Kim1, Byung Churl Lee2 |
1Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea
2Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea |
| 한국 소아 1형 당뇨병에서 종양괴사인자 및 림프독소-α 유전자 다형성 |
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서진순1, 박소영1, 정민호1, 서병규1, 김태규1, 이병헉2 |
1가톨릭대학교 의과대학 소아과학교실
2가톨릭대학교 의과대학 미생물학교실 |
Correspondence:
Byung Churl Lee, Email: byungcl@catholic.ac.kr
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| Abstract |
Purpose
: Recently, it was reported that tumor necrosis factor(TNF) and lymphotoxin-α(LT-α) gene regions might be a susceptible loci to type 1 diabetes in Japanese. The purpose of this study was to investigate the association of TNF and LT-α gene polymorphisms with disease susceptibility in Korean children with type 1 diabetes.
Methods
: Forty-nine Korean children with type 1 diabetes(29 girls and 20 boys) and 94 healthy Koreans were investigated in this study. Genotyping for -857T/C polymorphism in the TNF promoter region and LT-α gene polymorphism were performed by PCR-RFLP(restriction fragment length polymorphism). TNF promoter -1031C/T polymorphism was detected by allele-specific PCR.
Results
: The distribution of the -857T/C and -1031C/T genotype in the TNF promoter region was not different between diabetic children and the controls. The frequency of TT genotype in the distribution of TNF -1031C/T polymorphism in diabetic children with diabetic ketoacidosis(DKA) at diagnosis was significantly lower than those without DKA(P<0.05). No significant difference in the distribution of LT-α gene polymorphism was observed between diabetic children and the controls. There was no association between clinical characteristics of type 1 diabetes and LT-α gene polymorphisms.
Conclusion
: These results suggest that TNF promoter -857T/C and LT-α gene polymorphisms are not associated with susceptibility to type 1 diabetes in Korean children. TNF promoter -1031C/T polymorphism might be related to clinical manifestations(DKA) of type 1 diabetes. |
| Key Words:
Type 1 diabetes , Gene , Polymorphism , Tumor necrosis factor , Lymphotoxin-α |
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