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Purpose : The purpose of this study was to observe the effect of IFN-γ and PGE2 on TNF-α
production by human peripheral mononuclear cells(PBMC) that were stimulated with LPS.
Methods : PBMC were separated by Ficoll-Hypaque gradient centrifugation from human
peripheral venous blood and were incubated for 72 hours with or without LPS, IFN-γ, PGE2,
and indomethacin according to various conditions. TNF-α activity of... |