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Original Article
The Effect of IFN-γ and PGE2 in the Production of TNF-α by Human PBMC Stimulated with LPS
Youn Soo Hahn, Joong Gon Kim
Clin Exp Pediatr. 1996;39(2):255-264.   Published online February 15, 1996
Purpose : The purpose of this study was to observe the effect of IFN-γ and PGE2 on TNF-α production by human peripheral mononuclear cells(PBMC) that were stimulated with LPS. Methods : PBMC were separated by Ficoll-Hypaque gradient centrifugation from human peripheral venous blood and were incubated for 72 hours with or without LPS, IFN-γ, PGE2, and indomethacin according to various conditions. TNF-α activity of...