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Original Article

The Improvement of Cell Viability due to Dilution and Removal of DMSO in Thawing of Stem Cells

Hoon-Kyung Lee, Kyung-Ha Ryu, Il-Tae Whang, Eun Suk Kang, Ki Sook Hong, Kyung-Hyo Kim, Young-Mi Hong, Kyung-Hee Kim, Keun Lee

Clin Exp Pediatr. 2000;43(2):241-246. Published online February 15, 2000

Purpose : Cryopreserved stem cells from cord blood are usually infused with Dimethyl Sulfoxide (DMSO) immediately after thawing. However, this process may cause cell damage due to osmotic shock, and the administration of DMSO may also cause toxic effects. We studied a new method of increasing cell viability by stabilizing osmolarity by adding dextran 40 and washing out DMSO. Methods :...

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