Search

  • HOME
  • Search
Original Article
Enzyme Amplification, A Method Applied to Provide An ELISA for Studying Anti-Hib-Ps Antibodies in Children
Kyung Hyo Kim, Moon H. Nahm
Clin Exp Pediatr. 1997;40(2):173-180.   Published online February 15, 1997
Purpose : Enzyme immunoassay (EIA) is now a widely used technique. We have described the application of enzyme amplification (sensitive ELISA) in the field of immunoassays of pediatric population. There are two issues with the sensitive ELISA. First is that one can minimize the serum volume, an important concern for pediatricians. The second is the problem of background signal. We demonstrate that it...
Structure and Expression of Human Antibody Clones Specific for H. influenzae type b Capsular Polysaccharides
Kyung Hyo Kim, Gook Hyun Chung, Mitchell G. Scott, Moon H. Nahm
Clin Exp Pediatr. 1995;38(5):649-659.   Published online May 15, 1995
Purpose : Haemophilas influenzae type b is a significant pathogen in children and several v accines with different immunologic properties are clinically used. An individual produces oligoc-lonal anti-Hib-PS antibodies and the repertoire of human anti-HihPS Antibody (Ah) can be div- ided into two types by the structure of the light chain V region. Ah using A2-VK II gene product, which...