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Original Article
The Improvement of Cell Viability due to Dilution and Removal of DMSO in Thawing of Stem Cells
Hoon-Kyung Lee, Kyung-Ha Ryu, Il-Tae Whang, Eun Suk Kang, Ki Sook Hong, Kyung-Hyo Kim, Young-Mi Hong, Kyung-Hee Kim, Keun Lee
Clin Exp Pediatr. 2000;43(2):241-246.   Published online February 15, 2000
Purpose : Cryopreserved stem cells from cord blood are usually infused with Dimethyl Sulfoxide (DMSO) immediately after thawing. However, this process may cause cell damage due to osmotic shock, and the administration of DMSO may also cause toxic effects. We studied a new method of increasing cell viability by stabilizing osmolarity by adding dextran 40 and washing out DMSO. Methods :...
Respiratory Syncytial Virus Infections and Application of Nested Reverse Transcription-Polymerase Chain Reaction
Kon-Hee Lee, Hae-Sun Yoon, Kyu-Man Lee, Kyung-Hee Kim, Yang-Ja Cho
Clin Exp Pediatr. 1995;38(11):1486-1497.   Published online November 15, 1995
Purpose : Respiratory syncytial virus(RSV) in the most common cause of bronchiolitis and pneumonia in chileren less than 2 years old. Complications of RSV infections may be life-threatening in children who are immunosuppressed or who have congenital heart disease. Thus it is imperative that laboratory results be rapid and accurate. We therefore were interested in evaluating the role of RSV...