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Original Article
Periventricular leukomalacia induced by in utero clamping of pregnant rat aorta in fetal rats
Yun Sil Chang, Dong Kyung Sung, Saem Kang, Soo Kyung Park, Yu Jin Jung, Hyun Joo Seo, Seo Heui Choi, Won Soon Park
Clin Exp Pediatr. 2008;51(8):874-878.   Published online August 15, 2008
Purpose : This study was undertaken to develop an animal model of periventricular leukomalacia (PVL) induced by in utero clamping of pregnant rat aorta in fetal rats. Methods : A timed pregnanct Sprague-Dawley rat on embryonic day 21 just prior to delivery was sedated and anesthetized, and a Harvard ventilator for small animals was applied. Following laparotomy, the maternal aorta was...
Effects of NG-monomethyl-L-arginine and L-arginine on cerebral hemodynamics and energy metabolism during reoxygenation-reperfusion after cerebral hypoxia-ischemia in newborn piglets
Sun Young Ko, Saem Kang, Yun Sil Chang, Eun Ae Park, Won Soon Park
Clin Exp Pediatr. 2006;49(3):317-325.   Published online March 15, 2006
Purpose : This study was carried out to elucidate the effects of nitric oxide synthase(NOS) inhibitor, NG-monomethyl-L-arginine(L-NMMA) and nitric oxide precursor, L-arginine(L-Arg) on cerebral hemodynamics and energy metabolism during reoxygenation-reperfusion(RR) after hypoxia- ischemia(HI) in newborn piglets. Methods : Twenty-eight newborn piglets were divided into 4 groups; Sham normal control(NC), experimental control(EC), L-NMMA(HI & RR with L-NMMA), and L-Arg(HI & RR...
Single Cell Dissociation Methods for Flow Cytometric Cell Death Analysis of Hypoxia-Ischemia Injured Newborn Rat Pup Brain
Jong Hee Hwang, Dong Kyung Sung, Chang Won Choi, Saem Kang, Yun Sil Chang, Won Soon Park, Munhyang Lee
Clin Exp Pediatr. 2005;48(5):545-550.   Published online May 15, 2005
Purpose : Newborn brain tissue has to be dissociated into a single cell suspension for flow cytometric analysis of cell death during hypoxia-ischemia. Thus the development of a method to dissociate cells from the brain tissue with least damage and maintenance of membrane and antigen integrity remains the challenge for the in vivo application of this technique. We evaluated the...