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Original Article

Detection of Cytomegalovirus DNA in Urine Culture Using Polymerase Chain Reaction

Young Mo Sohn, Won Young Lee

Clin Exp Pediatr. 1993;36(5):626-633. Published online May 15, 1993

Polymerase chain reaction(PCR) amplication was used to detect cytomegalovirus (CMV) in tissue culture from the ruine of newborns and patients who was suspected CMV infection. Synthetic oligonucleotide primer pairs were used to amplify DNA from the magor immediate-early and the phosphoprotein 150 genes of CMV AD 169. Amplified products were detected by gel electrophoresis and by dot-blot hybridization with oligonucleotide...

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