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Original Article

The Effect of Testosterone on the Response of Grow Hormone Secretion in Cultured Rat Pituitary Cells

Ho Seong Kim, Duk Hi Kim, Deok Bae Park

Clin Exp Pediatr. 1993;36(11):1578-1582. Published online November 15, 1993

The effects of testosterone on the pituitary growth hormone(GH) response directly and to hypothalamic growth hormone-releasing hormone(GHRH) were evaluated in vitro using a male pituitary cell monolayer culture system. Wistar male rats were gonadectomized at 22 days of age, and 21 days later their anterior pituitaries were removed and trypsinized for cell dispersion. Testosterone 0.01, 1.0, 10.0nM was added to...

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