Clinical and Experimental Pediatrics

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Original Article

Domonstration of in vivo and in vitro Changes of T Helper Lymphocytes by Using Cytokine Flow Cytometry

Yeom Kim, Youn Soo Hahn

Clin Exp Pediatr. 1998;41(7):883-892. Published online July 15, 1998

The Effect of IFN-γ and PGE2 in the Production of TNF-α by Human PBMC Stimulated with LPS

Youn Soo Hahn, Joong Gon Kim

Clin Exp Pediatr. 1996;39(2):255-264. Published online February 15, 1996

Purpose : The purpose of this study was to observe the effect of IFN-γ and PGE2 on TNF-α production by human peripheral mononuclear cells(PBMC) that were stimulated with LPS. Methods : PBMC were separated by Ficoll-Hypaque gradient centrifugation from human peripheral venous blood and were incubated for 72 hours with or without LPS, IFN-γ, PGE2, and indomethacin according to various conditions. TNF-α activity of...

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