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Original Article
Changes in the expression of cytokines and apoptosis-related genes in children with infectious mononucleosis
Dae Sun Jo, Ji Hye Han, Sun Young Kim, Min Sun Kim, Ho Keun Yi, Dae-Yeol Lee, Pyoung Han Hwang
Clin Exp Pediatr. 2009;52(12):1348-1357.   Published online December 15, 2009
Purpose : The aims of this study were to identify the clinical characteristics and determine the changes in the expression of cytokines and apoptosis-related genes in children with infectious mononucleosis. Methods : Serological examinations of 15 pediatric patients diagnosed with infectious mononucleosis were performed prospectively. Peripheral blood from the patients was used to compare the composition of T cell subsets,...
Iron chelating agent, deferoxamine, induced apoptosis in Saos-2 osteosarcoma cancer cells
Eun Hye Park, Hyo Jung Lee, Soo Yeon Lee, Sun Young Kim, Ho Keun Yi, Dae Yeol Lee, Pyoung Han Hwang
Clin Exp Pediatr. 2009;52(2):213-219.   Published online February 15, 2009
Purpose : Iron is a critical nutritional element that is essential for a variety of important biological processes, including cell growth and differentiation, electron transfer reactions, and oxygen transport, activation, and detoxification. Iron is also required for neoplastic cell growth due to its catalytic effects on the formation of hydroxyl radicals, suppression of host defense cell activities, and promotion of...
Capsaicin induced apoptosis and the enhanced anticancer effect of anticancer drugs in cancer cells
Sun Young Kim, You Jin Lee, Eun Hye Park, Ho Keun Yi, Dae Sun Jo, Jung Soo Kim, Pyoung Han Hwang
Clin Exp Pediatr. 2008;51(3):307-314.   Published online March 15, 2008
Purpose : Capsaicin, the major pungent ingredient in red pepper, has long been used in spices and food additives. It has been recently shown to induce apoptosis in several cell lines through a not well known mechanism. The aim of this study was to investigate the apoptosis-inducing effect of capsaicin on gastric cancer cells, and to provide valuable information concerning...
The anti-tumor mechanisms of p53 through the regulation of expression and glycosylation of insulin-like growth factor binding protein-3
Sun Young Kim, Se Rim Kim, Jung Chang Lee, Ho Keun Yi, Dae Yeol Lee, Pyoung Han Hwang
Clin Exp Pediatr. 2006;49(4):431-438.   Published online April 15, 2006
목 적 : 새로운 암억제 유전자로 알려진 IGFBP-3의 주요 기능은 IGF-I과 IGF-II와 결합을 하여 IGF의 기능을 조절하는 IGF dependent mechanism과 IGFBP-3 자체가 IGF와 결합과는 무관하게 세포의 apoptosis를 유도하는 IGF independent mechanism이 보고되었다. 암억제 유전자 p53의 대표적인 항암기전의 하나는 직접 IGFBP-3의 발현을 증가시키며, 발현된 IGFBP-3는 암세포의 apoptosis를 유도시킨다. IGFBP-3의 항암작용은 보고되었지만, IGFBP-3의 변역 후 변형에 의한 항암기전은 전혀 밝혀져 있지 않다....
Case Report
A Case of Wiskott-Aldrich Syndrome with Novel Mutation in Exon 2 of the WASP Gene
Hyuk Lee, Jung In Park, Sun Young Kim,, Kyeung Hee Moon, Ho Keun Yi, Pyoung Han Hwang
Clin Exp Pediatr. 2005;48(5):551-556.   Published online May 15, 2005
Wiskott-Aldrich syndrome(WAS) is an X-linked recessive immunodeficiency characterized by thrombocytopenia with small platelet volume, eczema, and recurrent infections, and is also characterized by increased incidence of auto immune diseases and malignancies. The phenotype observed in this syndrome is caused by mutation in the Wiskott-Aldrich syndrome protein(WASP) gene localized to the proximal short arm of the X chromosome and recently isolated...
Original Article
The Tumor Suppressor Function of PTEN/MMAC1 through the Regulation of IGFs and IGFBPs
Ho Keun Yi, Dong Jin Hwang, Sun Young Kim, Dae Yeol Lee, Pyoung Han Hwang
Clin Exp Pediatr. 2004;47(8):884-891.   Published online August 15, 2004
Purpose : PTEN/MMAC1, a novel tumor suppressor gene, is mutated in a variety of advanced and metastatic cancers. It acts as a phosphatase, and thereby, regulates the PI-3 kinase/Akt pathway. In this study, we examined to evaluate the new function of anti-tumor effects of PTEN/MMAC1 through the regulation of the IGFs-IGFBPs in gastric cancer cells. Methods : PTEN/MMAC1 was expressed in...
Diagnostic Approach of Wiskott-Aldrich Syndrome
Dong Jin Hwang, Jun Won Yang, Sun Young Kim, Ho Keun Yi, Dae Yeol Lee, Pyoung Han Hwang
Clin Exp Pediatr. 2004;47(7):726-734.   Published online July 15, 2004
Objective : A probable diagnosis of Wiskott-Aldrich syndrome(WAS) should be considered in any boy presenting with unusual bleeding, congenital thrombocytopenia and small platelets. The definitive diagnosis of WAS is usually made by the detection of WASP gene mutation or a decrease or absence of the WAS protein(WASP) in blood cells using molecular genetic analysis. However, these methods are too time-consuming...
Apoptotic Pathway Induced by Dominant Negative ATM Gene in CT-26 Colon Cancer Cells
Jung Chang Lee, Ho Keun Yi, Sun Young Kim, Dae Yeol Lee, Pyoung Han Hwang, Jin Woo Park
Clin Exp Pediatr. 2003;46(7):679-686.   Published online July 15, 2003
Purpose : Ataxia telangiectasia mutated(ATM) is involved in DNA damage responses at different cell cycle checkpoints, and signalling pathways associated with regulation of apoptosis in response to ionizing radiation(IR). However, the signaling pathway that underlies IR-induced apoptosis in ATM cells has remained unknown. The purpose of this study was, therefore, to investigate the apoptotic pathway that underlies IR-induced apoptosis in...
Novel Gap Junction Molecules, Connexin 37, Enhances the Bystander Effect in HSVtk/GCV Gene Therapy
Sun Young Kim, Ho Keun Yi, Jung Chang Lee, Dong Jin Hwang, Pyoung Han Hwang, Dae Yeol Lee, Soo Chul Cho
Clin Exp Pediatr. 2003;46(6):541-547.   Published online June 15, 2003
Purpose : Gap junction intercellular communication(GJIC) is an important mechanism of the bystander effect in herpes simplex thymidine kinase/ganciclovir(HSVtk/GCV) gene therapy Therefore, we attempted to enhance the bystander effect in vitro by exogenous overexpressing connexin 37(Cx37) in cells to increase GJIC. Methods : NIH3T3 cells were transfected with the Cx37 and HSVtk gene or the HSVtk gene alone by the calcium...
iption Factor NF-κB Caused by Acute Hypoxia in the Heart
Chan Uhng Joo, Woo Suk Juhng, Jae Cheol Kim, Ho Keun Yi
Clin Exp Pediatr. 2002;45(9):1106-1113.   Published online September 15, 2002
Purpose : Nuclear factor-κB(NF-κB) is now recognized as playing a potential role in programmed cell death and the adaptive response to various stress. Cellular hypoxia is a primary manifestation of many cardiovascular diseases. It seems that vascular endothelial growth factor (VEGF) and insulin like growth factor-I(IGF-I) have a function as a protective molecule in the heart against several stress including...
Anti-angiogenesis and Anti-metastasis Characteristics of the Novel Tumor Suppressor Gene PTEN
Pyoung Han Hwang, Ho Keun Yi, Myoung Hee Han, Dae Yeol Lee, Dae Yeol Lee, Jung Soo Kim
Clin Exp Pediatr. 2001;44(6):654-663.   Published online June 15, 2001
Purpose : The tumor suppressor gene PTEN/MMAC1 is mutated in a variety of advanced and metastatic cancers, strongly suggesting that PTEN/MMAC1 alterations may be involved in tumor progression and the formation of metastases. However, the roles of PTEN in tumor growth and metastasis and its functional mechanisms are not fully understood. We evaluated the tumor suppressor function of PTEN/MMAC1 gene...
Studies on Functional Differentiation of Small Intestinal Epithelial Cells
Ho Keun Yi, Su Mi Oh, Dae Yeol Lee, Jung Soo Kim, Pyoung Han Hwang
Clin Exp Pediatr. 2001;44(5):523-532.   Published online May 15, 2001
Purpose : We investigated compounds from food sources given to children that may induce the differentiation of small intestinal epithelial cells in order to signal pathways that induce the proliferation and differentiation of small intestinal epithelial cells. Methods : We analyzed small intestinal epithelial cell differentiation using in vitro IEC-6 cells model. The growth curve of IEC-6 cells was obtained...
Induction of Apoptosis of Ewing`s Sarcoma Cells by Regulating Fusion Protein Expression
Ho Keun Yi, Ran Ju Kim, Dae Yeol Lee, Jung Soo Kim, Pyoung Han Hwang
Clin Exp Pediatr. 2000;43(7):942-951.   Published online July 15, 2000
Purpose : Fusion genes(EWS-Fli-1 and EWS-erg ) function as transcription activators and are essential for maintaining tumorigenic properties in Ewing`s sarcoma cells. Several reports have noted that Ets family transcription factors bind with CBP(CREB binding protein) in vitro. To understand the interaction of fusion proteins and CBP, we studied the CBP protein in TC135 cells expressing the EWS-Fli-1 gene. We...
Effects of Cefodizime on Phagocytosis of COS-1 Ccells
Pyoung Han Hwang, Sang Yun Nam, Ho Keun Yi, Min Ho Kim, Kong Soo Kim, Jung Soo Kim
Clin Exp Pediatr. 1998;41(12):1632-1638.   Published online December 15, 1998
Purpose : Cefodizime is a new third-generation cephalosporin which has a structure and immunomodutation properties similar to cefotaxime. Various studies on cefodizime have demonstrated the direct eradication of bacteria in cooperation with the host defense mechanism, particularly with phagocytosis. We evaluated the effects of cefodizime on the phagocytosis of COS-1 cells transfected with FcγRI/γγ or FcγRIIA cDNA. Methods : Phagocytosis was...